Primer Tm (Wallace / Nearest-Neighbor)
Calculate Primer Tm (Wallace / Nearest-Neighbor) instantly with the exact formula and a worked example. Free online calculator — no signup, works on mobile.
Primer Tm (Wallace / Nearest-Neighbor)
GC %
Length
Tm
68.25°C
More about: Primer Tm (Wallace / Nearest-Neighbor)
What it calculates
The “Primer Tm (Wallace / Nearest-Neighbor)” calculator computes Tm in °C from 2 parameters: gc %, length.
A lab calculation in biology and molecular biology.
Example calculation
With parameters GC % = 50, Length = 20 the result is 68.25 °C.
How to use
- Enter the parameter values — every field above is adjustable with a slider.
- The result and related metrics are calculated automatically as you type.
- Use the additional metrics shown (if any) where needed.
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