Protein Concentration
Calculate Protein Concentration instantly with the exact formula and a worked example.
Protein Concentration
Absorbance A280
Molar extinction coefficient εM⁻¹·cm⁻¹
Protein molecular weightDa
Path lengthcm
Dilution factor
Protein concentration
1.0005mg/mL
Molar concentration
15.06µM
A280 of a 1 mg/mL solution
0.66
More about: Protein Concentration
What it calculates
The “Protein Concentration” calculator computes Protein concentration in mg/mL from 5 parameters: absorbance a280, molar extinction coefficient ε (M⁻¹·cm⁻¹), protein molecular weight (Da), path length (cm), dilution factor.
A lab calculation in biology and molecular biology.
Example calculation
With parameters Absorbance A280 = 0.66, Molar extinction coefficient ε = 43,824 M⁻¹·cm⁻¹, Protein molecular weight = 66,430 Da, Path length = 1 cm, Dilution factor = 1 the result is 1 mg/mL (Spectrophotometer linear range — up to about A280 = 1.5).
How to use
- Enter absorbance a280, molar extinction coefficient ε, protein molecular weight, path length and dilution factor — each field above is adjustable with a slider.
- Protein concentration (mg/mL) is calculated automatically as you type.
- Check the worked example below to see the formula applied to real numbers.
- Copy the result or bookmark this calculator.
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FAQ
How is Protein Concentration calculated?
The Protein Concentration calculator computes Protein concentration (in mg/mL) from absorbance a280, molar extinction coefficient ε, protein molecular weight, path length, dilution factor. Enter your values above and the exact formula is applied instantly; a worked example with real numbers is shown below.
Is the Protein Concentration calculator free?
Yes. It is completely free, needs no signup, runs entirely in your browser, and sends no data to any server.
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