PCR Annealing Temperature
Calculate PCR Annealing Temperature instantly with the exact formula and a worked example.
PCR Annealing Temperature
GC %
Primer length
Tm
68.25°C
More about: PCR Annealing Temperature
What it calculates
The “PCR Annealing Temperature” calculator computes Tm in °C from 2 parameters: gc %, primer length.
A lab calculation in biology and molecular biology.
Example calculation
With parameters GC % = 50, Primer length = 20 the result is 68.25 °C.
How to use
- Enter gc % and primer length — each field above is adjustable with a slider.
- Tm (°C) is calculated automatically as you type.
- Check the worked example below to see the formula applied to real numbers.
- Copy the result or bookmark this calculator.
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FAQ
How is PCR Annealing Temperature calculated?
The PCR Annealing Temperature calculator computes Tm (in °C) from gc %, primer length. Enter your values above and the exact formula is applied instantly; a worked example with real numbers is shown below.
Is the PCR Annealing Temperature calculator free?
Yes. It is completely free, needs no signup, runs entirely in your browser, and sends no data to any server.
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