PCR Annealing Temperature
Calculate PCR Annealing Temperature instantly with the exact formula and a worked example. Free online calculator — no signup, works on mobile.
PCR Annealing Temperature
GC %
Primer length
Tm
68.25°C
More about: PCR Annealing Temperature
What it calculates
The “PCR Annealing Temperature” calculator computes Tm in °C from 2 parameters: gc %, primer length.
A lab calculation in biology and molecular biology.
Example calculation
With parameters GC % = 50, Primer length = 20 the result is 68.25 °C.
How to use
- Enter the parameter values — every field above is adjustable with a slider.
- The result and related metrics are calculated automatically as you type.
- Use the additional metrics shown (if any) where needed.
- Copy the result or bookmark the page.